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gapdh fitzerald industries international  (Bethyl)
96
Bethyl gapdh fitzerald industries international
Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; <t>GAPDH,</t> glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; <t>RPA32,</t> the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).
Gapdh Fitzerald Industries International, supplied by Bethyl, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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prox1 d2j6j antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc prox1 d2j6j antibody
Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; <t>GAPDH,</t> glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; <t>RPA32,</t> the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).
Prox1 D2j6j Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/prox1 d2j6j antibody/product/Cell Signaling Technology Inc
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prox1 d2j6j antibody - by Bioz Stars, 2026-03
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Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; RPA32, the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).

Journal: DNA repair

Article Title: Repair of protein-linked DNA double strand breaks: Using the adenovirus genome as a model substrate in cell-based assays

doi: 10.1016/j.dnarep.2018.12.001

Figure Lengend Snippet: Detection of covalent TP-oligonucleotide complexes during dl1004 infection. (A) Schematic of labeling assay. TdT, terminal deoxynucleotidyl transferase. (B) TP immunoprecipitated from dl1004-infected HeLa, U2OS, or HFFF cells can be labeled with [α-32P]-cordycepin triphosphate by TdT. The arrow indicates the location of “free” TP, as determined by immunoblotting. DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase. (C) Testing the presumed “TP-oligo” species for protein, DNA, and a serine phosphodiester linkage using Proteinase K, mung bean (MB) nuclease, or piperidine, respectively. (D) The mobility differences of the virus-specific, TdT-dependent cluster of TP bands are not due to differential phosphorylation. CIP, calf intestinal phosphatase; Na3VO4, the phosphatase inhibitor sodium orthovanadate; RPA32, the 32 kDa subunit of replication protein A; RPA32 (S4/S8), RPA phosphorylated at serines 4 and 8. (E) 2D gel analyses of TP-oligo from mock- and dl1004-infected HeLa cells. Isoelectric focusing is along the x-axis while conventional SDS-PAGE is along the y-axis. (F) Analysis of labeled DNA oligos that were released from immunoprecipitated TP by incubation in piperidine, by separation on a DNA sequencing gel. Numbers denote the location of reference oligonucleotides derived from the 5′ termini of the Ad dl1004 genome (which are identical to one another for the first 103 nucleotides).

Article Snippet: Additional antibodies used in this study: table ft1 table-wrap mode="anchored" t5 Protein Target Antibody Source Catalog # Adenovirus Gift from Arnold Levine, Institute for Advanced Study Monoclonal DBP #B6 GAPDH Fitzerald Industries International (RDI Division) RDI-TRK5G4- 6C5 RPA32 Gift from Thomas Melendy, State University of New York at Buffalo _____ RPA32 (S4/S8) Bethyl A300-245 A Nbs1 Novus Biologicals NB 100-143 Mre11 GeneTex GTX70212 CtIP Santa Cruz Biotechnologies sc-5970 BRCA1 Calbiochem OP92 HA-tag Santa Cruz Biotechnologies sc-7392 Open in a separate window 2.1.

Techniques: Infection, Labeling, Immunoprecipitation, Western Blot, Binding Assay, Virus, Phospho-proteomics, Two-Dimensional Gel Electrophoresis, SDS Page, Incubation, DNA Sequencing, Derivative Assay

Variation in TP-oligo size during dl1004 vs. WT Ad5 infection. HeLa cells were infected with dl1004 (A) or WT (B), harvested at the indicated time post infection, and TP was immunoprecipitated with the indicated antibody before being labeled with [α-32P]-cordycepin triphosphate by TdT. Nbs1, Nijmegen breakage syndrome protein 1 (Nibrin); DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; h.p.i., hours post infection.

Journal: DNA repair

Article Title: Repair of protein-linked DNA double strand breaks: Using the adenovirus genome as a model substrate in cell-based assays

doi: 10.1016/j.dnarep.2018.12.001

Figure Lengend Snippet: Variation in TP-oligo size during dl1004 vs. WT Ad5 infection. HeLa cells were infected with dl1004 (A) or WT (B), harvested at the indicated time post infection, and TP was immunoprecipitated with the indicated antibody before being labeled with [α-32P]-cordycepin triphosphate by TdT. Nbs1, Nijmegen breakage syndrome protein 1 (Nibrin); DBP, Ad DNA binding protein; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; h.p.i., hours post infection.

Article Snippet: Additional antibodies used in this study: table ft1 table-wrap mode="anchored" t5 Protein Target Antibody Source Catalog # Adenovirus Gift from Arnold Levine, Institute for Advanced Study Monoclonal DBP #B6 GAPDH Fitzerald Industries International (RDI Division) RDI-TRK5G4- 6C5 RPA32 Gift from Thomas Melendy, State University of New York at Buffalo _____ RPA32 (S4/S8) Bethyl A300-245 A Nbs1 Novus Biologicals NB 100-143 Mre11 GeneTex GTX70212 CtIP Santa Cruz Biotechnologies sc-5970 BRCA1 Calbiochem OP92 HA-tag Santa Cruz Biotechnologies sc-7392 Open in a separate window 2.1.

Techniques: Infection, Immunoprecipitation, Labeling, Binding Assay

2.1. Antibodies

Journal: DNA repair

Article Title: Repair of protein-linked DNA double strand breaks: Using the adenovirus genome as a model substrate in cell-based assays

doi: 10.1016/j.dnarep.2018.12.001

Figure Lengend Snippet: 2.1. Antibodies

Article Snippet: Additional antibodies used in this study: table ft1 table-wrap mode="anchored" t5 Protein Target Antibody Source Catalog # Adenovirus Gift from Arnold Levine, Institute for Advanced Study Monoclonal DBP #B6 GAPDH Fitzerald Industries International (RDI Division) RDI-TRK5G4- 6C5 RPA32 Gift from Thomas Melendy, State University of New York at Buffalo _____ RPA32 (S4/S8) Bethyl A300-245 A Nbs1 Novus Biologicals NB 100-143 Mre11 GeneTex GTX70212 CtIP Santa Cruz Biotechnologies sc-5970 BRCA1 Calbiochem OP92 HA-tag Santa Cruz Biotechnologies sc-7392 Open in a separate window 2.1.

Techniques: